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KMID : 0624620130460020124
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BMB Reports
2013 Volume.46 No. 2 p.124 ~ p.129
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Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model
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Kim Dae-Won
Lee Sung-Ho
Ku Sae-Kwang
Cho Soo-Hyun
Cho Sung-Woo
Yoon Ga-Hyeon
Hwang Hyun-Sook
Park Jin-Seu
Eum Won-Sik
Kwon Oh-Shin
Choi Soo-Young
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Abstract
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FK506 binding protein 12 (FK506BP) belongs to a family of immunophilins, and is involved in multiple biological processes. However, the function of FK506BP in corneal disease remains unclear. In this study, we examined the protective effects on dry eye disease in a Botulinum toxinA (BTX-A) induced mouse model, using a cell-permeable PEP-1-FK506BP protein. PEP-1-FK506BP efficiently transduced into human corneal epithelial cells in a time- and dose-dependent manner, and remained stable in the cells for 48 h. In addition, we demonstrated that topical application of PEP-1-FK506BP was transduced into mouse cornea and conjunctiva by immunohistochemistry. Furthermore, topical application of PEP-1-FK506BP to BTX-A-inducedmouse model markedly inhibited expression levels of pro-inflammatory cytokines such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and macrophage inhibitory factor (MIF) incorneal and conjunctival epithelium. These results suggest PEP-1-FK506BP as a potential therapeutic agent for dry eye diseases.
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KEYWORD
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Cytokines, Dry eye disease, Inflammation, PEP-1-FK506BP, Protein therapy
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